To assess the oxidative assaults and antioxidant defense, oxidative stress markers, including lipid peroxidation level, protein carbonylation level, Upholstered Arm Chair GSSG/GSH ratio and nonenzymatic antioxidants such as total glutathione, ascorbic acid and uric acid, in liver and brain tissues of hibernating common Asian toads, Duttaphrynus melanostictus, were compared with toads during active periods.Oxidative stress was found in both liver and brain tissues of hibernating common Asian toads in spite of depressed metabolism and low oxygen consumption.Significantly higher lipid peroxidation, protein carbonylation and an increased GSSG/GSH ratio were found in liver and brain tissues of hibernating toads, indicating oxidative stress.To counteract the stress, ascorbic acid was increased significantly in the liver and brain tissues of hibernating individuals in comparison to individuals during active periods.
The uric acid level decreased in both 3 GRAIN PANCAKE MIX the liver and brain tissues of hibernating toads, which may be due to its decreased rate of synthesis because of low xanthine oxidase activity at low body temperature and hypometabolism.The common Asian toad faced oxidative stress during hibernation, which was counteracted by augmented nonenzymatic antioxidant defense.